Is RNA polymerase saturated with substrates in vivo Describe

Is RNA polymerase saturated with substrates in vivo?

Describe experiments that might indicate whether RNA polymerase is operating at Vmax with respect to its nucleotide substrates.

Solution

First we have to know whether RNA polymerase saturated with substrates in vivo or not, we propose that this very design causes the cell to become subsaturated with precursors and catalytic components at all levels of macromolecular biosynthesis and leads to a molecular sharing economy at a high level of competition inside the cell the  amount of free RNA polymerase and the ribosome binding sites on the mRNA chains compete with each other for the free ribosomes.

The main disadvantage is the bacterial physiology is how bacteria adapt and optimize their rate of growth in response to different environments

The number of ribosomes per amount of total protein present in a growing culture of Escherichia coli increases in nearly direct proportion to the growth rate.

The rate of r-protein synthesis is negatively controlled by free r-proteins, in that excessive accumulation of free r-proteins causes a reduction in the translation and lifetime of r-protein mRNA and thereby reduced synthesis r protein.

The synthesis of r proteins to the synthesis of rRNA. For this reason, the study of the control of ribosome synthesis centers on the control of rRNA synthesis.

These directly interacting factors and effectors have been clearly identified can one begin to clarify the mechanisms by which the composition of the growth medium affects the activity of these factors.

any factor that affects these interactions can be defined and measured as a change in the Michaelis Menten parameters Vmax and Km.

To determine values for these parameters requires a systems biology approach, i.e., the use of mathematical tools to integrate experimental data into a logically consistent conceptional framework. various models for the control of rRNA synthesis in E. coli which have been proposed over a period of 45 years.This part begins with a description of the theory of transcript initiation, which forms the basis for our kinetic approach to the question of rRNA control. an evaluation of the gene activity data in terms of the kinetic properties of rrn promoters, and the conclusions from these studies with regard to the control of rRNA synthesis by ppGpp.The rate of rRNA transcription is positively controlled by one of the r-proteins and is thereby adjusted to the rate of synthesis of the primarily controlled r-protein and,r-protein promoters are constitutive.