Solution of specific vitamin has Lambdamax 251 nm and a conc

Solution of specific vitamin has Lambda_max =251 nm and a concentration of 2.08 Times 10^2 M. The absorbance of the solution at 251 nm is A = 0.160. What is the molar absorptivity of the vitamin at 251 nm? (sample pathlength = 1.00 cm)

Solution

A = absorbance , e = molar absorptivity, c = concentration, l = pathlength

From Beer-Lambert Law , A = e c l

e = A / c l

= 0.160 / (2.08 * 10^-7 * 1 cm)

= 7.69 * 10⁵ L mol^-1 cm^-1