please answer the following with regards to PCR please answe

please answer the following with regards to PCR:

please answer all 3 questions by choosing the correct answer thank you!

What happens in the reaction when the temperature shifts to 55 Cduring cycling? Select one: a. the DNA polymerase will carry out DNA synthesis by extending the annealed primers b. the DNA polymerase will finish DNA synthesis X c. the primers anneal to the single-stranded regions of the DNA d. the primers anneal to the double-stranded regions of the DNA. During cycling, what occurs when the temperature is at 72 C? Select one: a. the primers anneal to the double-stranded regions ofthe DNA b. the DNA polymerase will carry out DNA synthesis by extending the annealed primers c the primers anneal to the single-stranded regions of the DNA X d. the DNA polymerase will finish DNA synthesis You designed a set of primers to amplify 1 kb DNA with polymerase chain reaction. By which cycle of polymerase chain reaction, we would expect to see the first amplified DNA with expected size? Select one: a. The end of cycle 1 b. he end of cycle 2 The end of cycle 4 X d. The end of cycle 10

Solution

Answer1. The DNA polymerase will carry out the DNA synthesis by extending the annealed primers.

Answer 2. The primers anneal to the double strand regions of the DNA

Answer 3. By the end of cycle 10