What vector would you use to clone A a small amount of DNA B

What vector would you use to clone A) a small amount of DNA B) Several genes or a large DNA fragment C) an entire chromosome, to be cloned into a eukaryotic cell. What technique would you use to see if a particular sequence is A) on a fragment of DNA? B) present in a sample where it may be at a very low concentration? Name two techniques that can be used to study RNA concentration. You are trying to locate a gene on a plasmid. To do so, you cut it with restriction enzymes, and then run a gel electrophoresis and a Southern Blot. Draw the plasmid map, indicating where your gene of interest is, where each fragment is (Show the length of each one) and where each cut site is given the following information:

Solution

Clonning Vector is small piece of DNA, taken from a virus, a plasmid or the cell of higher organism that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.

Types of cloning vectors:

1. Plasmid: Cloning limit: 100 to 10,000 base pairs.

2. Phage vector: Clonning limit: 8-20 Kb

3. Cosmids: Clonning limit: 35-50 Kb

4. Bacterial artificial chromosome: Cloning limit: 75-300 Kb

5. Yeast artificial chromosome: Cloning limit: 100- 1000 Kb.

2. A southern blot is a method used in molecular biology for detection of specific DNA sequence sin DNA sample.

3. The techniques to study RNA concentration are Northern blotting and Fluorescent in situ hybridisation.