The first question was Aprrox how many copies of a 6 base pa

The first question was Aprrox how many copies of a 6 base pair restriction enzyme site (ex ACCGT) would you predict to be present in a piece of DNA that is 35000bp in length?

The answer is since there is only 1 Nsil restriction site present in 540 bp of circle DNA, there would be about 65 restriction sites in 35000 bp of DNA.

Now, I need this question answered......

How many fragments would be produced if the DNA were in linear form? In this scenerio, what would be the size (in bp) largest fragment produced

5\' ATG GGATCCG TTCAAGGcTT TGGAGATOTG TACTGTAGAG Tc GCT TCT AGGCCT TAAG HindIII CTAAATCAAA ATTTAAGTTT AGAAGCTAGG CCGGATCCCA GcGAT Hind GTACTGAT TGGACAAAAG CATTGATTAT AGTTGAAATT GATGcTAAAC CATTTGCAAA AGGTGCACTT AG TGCAT ATCAA TTGAA AATTOGATOT AATGCAATGc AATGTTTTAA TCATTTcGCA TAG AGTCGCT TTOTATAT T ACAAAATCAA CAACAAAAcc AACAACAACA. AAA CCAACAA CAACAACAAG AAGAAATTAA TTTTATAACA ACTGAAGATA AATTGcCAAAT TTACCAGATT CAAATT GTCA ATGGGCAATA ATTTGGGAAT ATTCAGCAAA TGATGATGAA ACACCAATTC ATGAGAA ATA TATGGAAGGA AAGAAATTAA ATTTATCACA AATTcCAAAA CTCACAT CTC AGGCTTTTAG AGTCCGAAAC CTCTAGA CAT GAccCAGA-3\'

Solution

If it is a circular DNA, only one fragment would be formed to open the circle - a large fragment of 540 bp

if it is a linear DNA, two fragments would be formed - largest being 345 bp.