A cell population that you are growing in a culture dish exp

A cell population that you are growing in a culture dish expresses Sox-2. How do you make sure it is a pluripotent stem cell and not a neural stem cell?

Solution

Induced pluripotent stem cells (iPSCs): A cell population that you are growing in a culture dish expresses Sox-2 7 these cells produced from adult cells, which reprogrammed to produce an embryonic stem cell through genetic reprogramming. There are total 4 potential factors responsible to transcribe and to induce these pluripotent stem cells. The following are Yamanaka factors, which have ability to induce pluripotent stage to fibroblast. 1. Oct 3/4, 2. Sox2; 3. C-myc; 4. Klf4. The SOX-2 levels are confirmed via SOX2-GFP expression during neural differentiation whereas the neural-specific marker PAX6 is going to higher in NPC & neural stem cell formation. These are not going to be higher in a culture dish expresses Sox-2 in a pluripotent stem cell so that it can be confirmed via flow cytometry & SOX2-GFP expression & fluorescence analysis