You did 5 fold dilutions and with the aid of an assay you we

You did 5 fold dilutions and with the aid of an assay you were able to estimate that the concentration of antibody specific for the H1N1 virus (Influenza) in the 7^th dilution tube is 5 ng/1 ml

a). Calculate the concentration, in mg/ml, of anti H1N1 virus antibody present in the serum of an infected individual employing the “Dilution Factor”. (Consider the serum as your “stock”)

Solution

Answer:

Given: concentration of H1N1 virus in the 7th dilution tube is 5 ng/1 ml

Each tube is 5 fold dilutions

Dilution factor in 7th tube is = x x x x x x =1/78125

Concentration in original stock = 5 ng/mL x 78125 = 390625 ng/mL

a). Calculate the concentration, in mg/ml,

1000000 ng ---->1mg

390625 ng/mL =0.390625 mg/mL