When utilizing immunocytochemistry to locate cell bodies of

When utilizing immunocytochemistry to locate cell bodies of a particular peptide-containing neuron, colchicine is often administered in order to increase the amount of reaction product in the cell bodies. Why would this manipulation be effective? Both excitatory and inhibitory synapses are present in the cerebellum. How would you prepare tissue to visualized synapse and what identifying characteristic would you looks for to differentiate the two types of synapses? Where in the cerebellum might you expect to find the different types of synapses?

Solution

Ques-2:

Normally, colchicine is going to increase cell bodies volume in a neuron and alters brain physiology. The colchicines pre-treatment of the neurons (specifically smaller neurons in amygdale) prior to immunohistochemistry is going to provide fluorescent signal confined to the cell bodies when utilizing immunohistochemistry to locate cell bodies of a particular peptide-containing neuron. The colchicine pre-treatment colchicine is often administered in order to increase the amount of reaction product in the cell bodies so that it is possible to examine the effective pattern of hrGFP fluorescence under the imaging finally it is possible to estimate the proper “co-localization of protein by immunohistochemical staining. In neurons