Calculate the pH for a 100 mM solution of strong acid Calcu

Calculate the pH for a 100 mM solution of strong acid. ___ Calculate the pH for a 100 mM solution of strong base. ___ A 1 mM weak add solution should have a pH value greater than ___ but less than ____.How many grams of sodium hydroxide (NaOH) would be required to prepare a liter of 0.63 M NaOH? (show your work) List the categories of biomolecules (also called macromolecules). What instrument is used to detect the amount of protein in a sample and how does it work? How many molecules are in 2 ml of 20 muM bovine serum albumin (BSA)? (show work)

Solution

1. Strong acid = H⁺

Eg: pH of 100mM of HCL

100mM = 0.1 M and pH = H⁺

So pH of 100mM (0.1M) = - log (H⁺)

= - log (0.1M)

=1

2. Strong base = OH^-

Eg: pH of 100mM of NaOH

i.e pH = OH^-

14 (-1) = 13

5. A Biomolecule is a molecule that is present in living organisms may be inherited or can be produced or synthesised by the body. There are four categories of carbohydrates:

a) Nucleic acids (includes both DNA and RNA)

b).Carbohydrates

c). Proteins and

d). Lipids

6. Protein Mass Spectrometer is the instrument used to detect the amount of protein in a sample.

Working Principle: Mass spectrometry is a central analytical technique for protein research and for the study of biomolecules in general.The foremost step in this technique is the production of gas phase ions for the given compound. This undergoes fragmentation, results in the yeilding of a radical with odd number of electrons and also an ion with an even number of electrons. These ions are seperated in the mass spectrometer according to their mass to charge ratio.

7. 1L = 20 X 10⁶ M (since 1 mole = 6.023 X 10²³)

Therefore, 2/1000 X 20 X 10⁶ X 6 X 10²³

= 0.002 X 120 X 10²⁹

= 0.24 X 10²⁹