Can someone please explain how to use mutations from a compl

Can someone please explain how to use mutations from a complementation table to determine the order of genes in a chromosome?

ge orm arch 3, 2016 Exam 2 gene to pair wise were performed and isolated in the deletion table. the ability of the following was In the recombine to give wild-type producedi wild-type bacteriophage means no wild-type bacteriophage recovered 1 3 4 5 Three new rl/A point mutants (f g and h) are isolated and crossed to all of the deletion Mutant f fails to recombine with deletions 2 and 5; mutant g fails to recombine with 4, 5 and 1; and mutant h fails to recombine with deletions 1 and 3. deletions Which point mutant is in the middle of the other two? A. Mutant f B. Mutant g C. Mutant h

Solution

Mutant f: fail to recombine with deletions 2 and 5. Can recombine with 1,3 and 4.

Mutant g: fail to recombine with deletions 1,4 and 5. Can combine with 2 and 3.

Mutant h: fail to recombine with 1 and 3 but can combine with 2, 4 and 5.

Therefore, mutant f is first in the order, mutant g is second in the order and mutant h is thrid in the order.

Mutant f - mutant g - mutant h. The mutat g is the point mutation between mutant f and mutant h. Option B is correct.