cateogorize the statement below as true or false to review t
cateogorize the statement below as true or false to review the characteristics of DNA replication.
a. the area of DNA that is unwound by DNA helicase is called a replication bubble and it contains a single replication fork.
b. Pol III can copy only unwound DNA; it can add nucleotides only to the end of an existing chain, and it can move in only one direction.
c. the lagging strand is synthesized in teh 5\' to 3\' direction, but this directionality results in okazaki fragments
d. A new DNA strand is always synthesized in teh 5\' to 3\' direction
e. An RNA primer is neccessary to terminate DNA replication at the telomere
. Okazaki fragments must be joined together by DNA polymerase III ( pol III).
g. An RNA primer is necessary to initate DNA replication by pol III
h. each replication bubble contains two replication forks, each of which is the only point at which the two DNA strands are unwinding from each other.
I. the leading strand is synthesized in the 5\' to 3\' direction, but the lagging strand is synthesized in the reverse sequence.
J. okazaki fragments must be joined together by DNA ligsae.
Solution
a. the area of DNA that is unwound by DNA helicase is called a replication bubble and it contains a single replication fork. ....TRUE
b.Pol III can copy only unwound DNA; it can add nucleotides only to the end of an existing chain, and it can move in only one direction....FALSE (it has both 5\'3\' polymerase and 3\'5\' exonuclease activity).
c.the lagging strand is synthesized in teh 5\' to 3\' direction, but this directionality results in okazaki fragments... TRUE (dna is always synthesised in 5\'3\' direction and cannot be synthesised inreverse direction)
d. A new DNA strand is always synthesized in teh 5\' to 3\' direction ...TRUE AS ABOVE
e. An RNA primer is neccessary to terminate DNA replication at the telomere.... false.
. Okazaki fragments must be joined together by DNA polymerase III ( pol III).... FALSE becoz okazaki fragments are joined by DNA ligase
g. An RNA primer is necessary to initate DNA replication by pol III... TRUE
h. each replication bubble contains two replication forks, each of which is the only point at which the two DNA strands are unwinding from each other. ....TRUE
I. the leading strand is synthesized in the 5\' to 3\' direction, but the lagging strand is synthesized in the reverse sequence.... FALSE becoz dna is always synthesised in 5\'3\' direction and cannot be synthesised in reverse ....
J. okazaki fragments must be joined together by DNA ligsae.... TRUE
BE HAPPY AS PER POLICY I HAD TO EXPLAIN ONLY FIRST FOUR PARTS ....BUT I TRIED TO ANSWER ALL ,,, HOPE IT HELPS .... REGARDS
