The from the bowman of the objective lens in the pelican is
Solution
1. The distance between the bottom of the objective lens and the specimen is called working distance.
2. The resolution of human eye or naked eye is 55-75 micrometers.
3. The area of the specimen seen when looking through the microscope is the field.
4.Ocular lens= 10x
Total magnification= 950x
so, 950x= 10x X objective lens
objective lens= 95x
5. Light should be dimmed when seen through the living cells because it may damage the cells.
6. If after focussing a low power only the fine adjustment need to be used to focus the specimen at the higher power, the microscope is said to be PAR FOCAL
Multiple choice
1. The resolution of microscope is increased by using blue light filter and rising the condenser to its highest point. Purpose of the condenser lens is to focus the light on to the specimen.Lowering the condenser demnishes the illumination and rising will increase the illumination.
2. Total magnification= Objective lens x Ocular lens
so, for 10x objective lens and 10x ocular lens total magnification= 10x X10x
= 100x
so, answer is 5. None of the above.
3. The most commonly used ocular is 10x. because it allow for highest magnification. So answer is 2.
4. The microscope lenses may be cleaned with lens tissue. Because dust formed on the lens needed to be removed to give the clear image. And we can also some solvents like alcohol, ether and rubber bulbs. So answer is 1.
5. When changing from low power to high power it is necessary to close the diapharm. so answer is 2. when close the diapharm resolution is less but depth of field is more.
Completion
1. Three lens systems in a compound microscope are
i) Eye piece or ocular lens
ii) Objective lens
iii) Condenser lens
2. The characterstic of a microscope that enables you to switch from one objective to another without altering the focus is called par focal.
3. In general, condenser should kept at as low as posiible to prevent damage to the lower lens.
True- false
1. True. Eye pieces had simple construction and can easily be removed to clean the lenses.
2. False. Lenses should clean with lens tissue paper especially made for microscopic lenses.
3. True. High- dry is 400x lens. oil immersion is a 100x lens. it should maintain distance between lens and specimen to avoid damage to the lens and to the specimen.
4. False. oil immersion used for when the object shows the low resolution with objective lens. so it not necessary to use low magnification lens before the oil immersion.
5.True. Working distance is the distance between the objective lens and the specimen.so its true.
6. True. oil immersion refractive index is 1.515 and glass is 1.52.
7. True. Generally oil immersion used when the object shows the low resolution with the objective lens and to decrease the light refraction.
8. True. Blue light filter gives better resolution than the white light.

