Using only methods described in MCDB 310 and all the informa

Using only methods described in MCDB 310 and all the information above, devise a method to purify the DNA binding protein from E. coli lysate (i.e. broken up bacteria in a buffer solution). You may only use two chromatography methods (not just two steps) for purification from the lysate, and you need the purest sample of the protein possible. Because this protein is newly discovered, there is no antibody available. Again, you already have lysed cells.

Methods described : Column chromotography - Separation by charge/ affiinity/ size

4. (2 points) Using only methods described in MCDB 310 and all the information above, devise a method to purify the DNA binding protein from E. coli lysate (i.e. broken up bacteria in a buffer solution). You may only use two chromatography methods (not just two steps for purification from the lysate, and you need the purest sample of the protein possible. Because this protein is newly discovered, there is no antibody available. Again, you already have lysed cells.

Solution

1.The first method to use will be cation exchange that includes separation by charge.In cation exchange ,the protein will bind to the anionic resin.

2.The protein can be further purified by affinity chromatography using a DNA column to which the protein will bind.