Using a heat fixed smear add the primary dye and stain for

Using a heat fixed smear, add the primary dye ______ and stain for ten minutes using heat. Rinse with ____. At the end of this step all organisms on slide will be stained the color_____. Add the decolorize ______ until color stops lifting freely off the slide. Rinse. At the end of this step acid fast organisms will be the color_______ and non-acid fast organisms will be the color. ______ Add the counter stain ______ for two minutes. Rinse. At the end of this step, acid fast cells will be the color _____ and non-acid fast organisms will be the color _____.

Solution

(a)Carbol fuchsin

(b)clean water

(c)Red

(d)Acid alcohol (3% HCL in 95% alcohol)

(e)Red

(f)colorless

(g)Methylene blue

(h)Red

(i)Blue