Thank you A capsule is made of fatty acids and protein and p

A capsule is made of fatty acids and protein and provides an area around the bacterium that can attract water. Bacillus species and Escherichia coli hold the safranine during the alcohol wash.

Solution

1) True: A bacterial capsule is the outside layer of the cell which is also called as the cell envelope of the bacteria. It can be found in both gram-positive and gram-negative bacteria. It is usually made up of polysaccharides, also consists peptidoglycan and muramic acid. The capsule takes a lighter stain than the bacteria and its background during any microscopic observation. As it is tightly packed it is difficult to stain the capsule.

2) False: Bacillus species and E coli are gram positive bacteria and the gram positive bacteria always retains the primary stain i.e. crystal violet and will not absorb the secondary stain i.e. safranine and hence gives a violet or purple color stain when viewed under the microscope. and if the bacteria is a gram negative bacteria then it retains the secondary stain and loses the primary stain and appears as red when viewed under the microscope.