Assuming you could use primers of four nucleotides write the

Assuming you could use primers of four nucleotides, write the sequences of the two primers (specify the 5\' and 3\' ends) that should be used to amplify the segment of DNA shown below by polymerase chain reaction - only one strand of the double-stranded DNA is shown. 5\'-G-A-T-T-C-A-G-C-T-A-C-C-G-G-A-C-G-A-T-C-G-C-T-A-G-C-3\'

Solution

Answer :

Given DNA sequence :     5’ G-A-T-T-C-A-G-C-T-A-C-C-G-G-A-C-G-A-T-C-G-C-T-A-G-C 3’

Complimentary sequence :    3’ C-T-A-A-G-T-C-G-A-T-G-G-C-C-T-G-C-T-A-G-C-G-A-T-C-G-5’

Primers

  5’ G-A-G-G 3’ (/Forward primer)

5’ G-C-T-A 3’ (Reverse primer)

After binding

5’ G-A-T-T-C-A-G-C-T-A-C-C-G-G-A-C-G-A-T-C-G-C-T-A-G-C 3’

3’A-T-C-G 5’

5’ G-A-G-G 3’

3’ C-T-A-A-G-T-C-G-A-T-G-G-C-C-T-G-C-T-A-G-C-G-A-T-C-G-5’