Identify the following method for physically mapping genes H

Identify the following method for physically mapping genes: Human and mouse cells are fused. The resulting hybrid cell randomly loses human chromosomes and retains only a few. A panel of hybrids that retain different combinations of human chromosomes is tested for expression of a human gene. A correlation between the expression of the gene and the retention of a unique human chromosome in those cell lines indicates that the human gene must be located on that chromosome. Deletion mapping Somatic cell hybridization In situ hybridization DNA sequencing Lod-score analysis

Solution

The answer is Somatic cell hybridization

human fibroblast cells and mouse tumor cells used for SCH at first time. Sendai Virus was used as a hybrizier, human fibroblast cells and mouse tumor cells grown together in culture will fuse to form a heterokaryote, in which the nuclei fuse. Because the cell machinery is primarily murine, over successive mitotic divisions, human chromosomes are gradually lost. Eventually, a number of hybrid cell lines are formed that have only one or a few human chromosomes, which are readily identifiable. These cell lines can be screened for the presence of various human biochemical markers, which are then assignable to the retained chromosome.