Please eaxplain a stepbystep method for the purification of
Please eaxplain a step-by-step method for the purification of water that uses PFA fixation?
Solution
1- Add deionized water to a flask and heat to 60 degrees.
2- Add PFA with constant stirring( in 1lit of final reaction volume,add 40g of PFA,water 750 ml,250ml of 0.4M buffer)
3-slowly add a few drops of 10N NaOH ,with stirring.
4- Remove from heat and cool to room temperature.
5- Filter with 3 whatman filter paper.
6-Add 0.4M Sodium phosphate buffer
7- Add 100 mls of distilled water.
8- Add 1M HCl to adjust ph 7.4-7
9-Bring to final volume with deionized water.

