Write IN DETAIL use pictures the lab procedure to show pglo

Write IN DETAIL (use pictures) the lab procedure to show pglo expression in eukaryotic cells using transfection process

Solution

Green fluorescent is a protein that glow on exposure to UV light PGLO plasmid contain GEP gene.For Transfection delivery into eukaryotic cell.Cell is grown in growth medium and kept in CO2 incubator for 24 hours Cell is grown in appropriate medium with growth factor . Passage it after 24 hours. Plating is done in six well plate transforming agent is introduced and kept in CO2 incubator and seen under UV light. Cell with GEP gene will grow brilliant green when exposed to UV light.