Prompt You have isolated what appears to be an intracellular

Prompt: You have isolated what appears to be an intracellular parasite from a blood culture from a human case of chronic fatigue syndrome. Electron microscopic studies indicated that the isolate looks like a virus, but the results are not conclusive. Your virology/bacteriology training makes you believe that this isolate is either a new virus or a protozoan parasite. Your virology professor tells you that your isolate is most likely a virus.

Assume that you have many eggs, and you can grow this pathogen in large quantity and purify it with ease. How would you go about to establish conclusively that your new isolate is a virus and why? Please explain in detail and your reasoning.

Solution

Cultivation :As we know that it can be cultured in embryonated eggs or tissue culture system or a whole animal system. The purpose of the cultivation is to isolate and identify the virus viral growth can cause cytopathic effects in cell culture these cytopathic effects cytocidal or non cytocidal where as the uninfected cells form a monolayer CULTURING: the tissue is trated with the enzymes to seperate the cells and the cells are suspended in the culture medium .the normal cells or the primary cells grow in monolayer across the container where as transformed cells donot grow in monolayer and form as seperate then those transformed cells should be isolated. after isolation the viral identification is done by :1.serological methods like westren blotting 2.molecular methods like pcr and RFLC\'s.