A student is carrying out sitedirected mutagenesis to introd

A student is carrying out site-directed mutagenesis to introduce a lysine residue that can form a salt bridge with Glutamate residue in a globular protein to stabilize the folded state. Calculate the maximum distance between the two charged residues to stabilize the protein at 37^o Celcius. (state any assumptions you make).

Solution

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The distance is 0.5 angstrom(A) .We will have the four distance data which will be arranged in a 4-D histogram.This will allow each contact pair to be binned at a resolution of 0.5 Å, from 0 to 10 Å.This forms histograms of 21⁴ bins which is 194481). The total distance = 0 +1/2=0.5 in all the 4 directions.