lane L ane Lane Lane Lane Lane 4 2686 SolutionThe only logic

lane L ane, Lane Lane Lane Lane 4 2686

The only logical explanation is that you have interchanged the lanes. The band in lane 3 is exactly 2686bp. You must have loaded The undigested vector in lane 3. Please repeat the experiment once again to confirm. The only other explanation is that there could be a contamination. In either case repetition is a must to validate the result.