You spin down 145 mls of cells to freeze While the cells are
You spin down 14.5 mls of cells to freeze. While the cells are spinning you count the cells and determine that there are 230,000 cells/ml, and that the cells are 98% viable. You need to resuspend your cells at a concentration of 2 times 10^6 cells/ml in freezing media. How much freezing media will you need?
Solution
some more nformation needed here,
according to Q, stock solution concentration of viable cells is 225400 cells/ml, final concentration needed is 2000000 cells / ml which is higher than the stock solution, diluted concetration must be less than that of stock solution.
