Biochem please help am so lost Ill rate The following sequen

131 140 170 190 VOSRROPPEEVOVL MSTFGYNI AINR EILERRESEGGIWOLFR EGETINTRRI ATRITL EIE RTRITL RINA EIE VIGSE d..E 410 430 Ar-Ivno RNIGORRWYRLsao----------------------- KNWLRONINYSRCual----------------------------- 690693 620 210 EQIREIKROTORINE TIILORSPITIRSI- PS ITIVSQ------PDRDGIRE 510 WRONGTWISTL .ple..T 610

Solution

Answer:

Baeyer–Villiger monooxygenases (BVMOs) form a distinct class of flavoproteins that catalyze the insertion of an oxygen atom in a C–C bond using dioxygen and NAD(P)H.

Ar-BVMO is a recently discovered Baeyer-Villiger monooxygenase from the genome of Acinetobacter radioresistens Experiments performed by Site-directed mutagenesis decipher the role of the active site arginine-292.

Replacement of arginine 292 in Ar-BVMO by glycine or alanine resulted in greatly reduced when by glycin and no Baeyer-Villiger activity when replaced by alanin,

The active site Arginine 292 is required for its Baeyer-Villiger activity but not for heteroatom oxidation.

A single mutation converts Ar-BVMO to a unique S- or N-monooxygenase, a useful biocatalyst for the production of oxidized metabolites of human drug metabolizing enzymes.