i am having conflicting answers between option A and C but w

i am having conflicting answers between option A and C, but which one is coreect?

Alteration in membrane asymmetry is employed for detection of early apoptosis. Interaction of which molecules is utilized in this process?

A. Phosphatidylserine and annexin-V B. DNA polymerase and dUTP C. Caspase 3 and cytochrome D. MTT and NADH

Solution

Answer is

A. Phosphatidylserine and annexin-V

During apoptosis, you can see the loss of the strong plasma membrane asymmetry existing in living cells and this loss of symmetry you can detect by using the probes which can interact strongly and specifically with phosphatidylserine (PS).

During apoptosis as this phospholipid becomes exposed to the cell surface, with the help of labeled annexin V you can easily detect them.

Spectroscopy of cell suspensions, microarray technology, flow cytometry and confocal or two-photon microscopy are used to this early and Ca2+-independent detection of apoptosis.

The exposed phosphatidylserine (PS) will produce integrated changes of electrostatic potential and hydration in the outer leaflet of cell membrane, your detection will become easy by direct response of smart fluorescence probes.