Luria broth agar plates were prepared using 15 agar The liqu

Luria broth agar plates were prepared using 1.5% agar. The liquefied medium was poured into sterile plates and allowed to solidify at room temperature. A bacterial culture was streaked out onto the plates and incubated at 65 C. At this temperature, will the medium remain solid or liquefy, please EXPLAIN?

Solution

The melting point of agar gel is approximately 85 degrees, so it remain solid at temperatures below 85 degrees. Thus, the agar remain in solid state at a temperature of 65 degrees.

Agar gel is used in concentrations 1 to 2% in bacterial culture media as a solidifying agent and as a source of carbon.