WOULD YOU CONSIDER AN EIA OR WESTERN BLOT AS BEING A MORE SE

WOULD YOU CONSIDER AN EIA OR WESTERN BLOT AS BEING A MORE SENSITIVE ASSAY? ,EXPLAIN YOUR REASONING.

Solution

Enzyme Immunoassays and Western blotting both are the sensitive technique of detecting proteins in the sample, EIA, is ELISA enzyme linked immuno sorbent assay which detect the antigen by treating with primary and secondary antibodies, whereas western blot is seperate proteins are blot transferred to nylon membrane and detected by antibodies for the pathogen determinant.

ELISA is the quantitative , western blot is qualitative analysis.these techniques are rather more sensitive because the antibodies uses is very specific for antigen.chemiluminiscence or flourescent. And enzyme linked for the secondary antibody.

Due to its sensitivity nature of detection it is used to detect even small proteins which are hard to isolate even can detect by the Western blotting technique( eg clock proteins)