how can I determine the dna concentration in a hazelnut cook

how can I determine the dna concentration in a hazelnut cookie?

Can I do it with a qPCR or droplet PCR? Do I first have to remove the matrix effect of sugar and fats? Do I first have to check the DNa integrity?

Solution

The best method to determine the DNA Concentration in a hazelnut cookie is to evaluate the DNA size distribution on agarose gel and maximum DNA dilution that can be done by amplification by the PCR. If you want the DNA concentration to be accurate go with qPCR than the droplet PCR. Yes, it is necessary to remove the matrix effect of sugar and fats otherwise the DNA extracted will have contamination and you will require to purify it. Yes, you should go for the check of the integrity of the DNA sample extracted from the cookie before estimation to check the level of degradation. If the sample extracted have the fragments larger than the predicted size then its fine otherwise you have to check the integrity of the sample.