Match the descriptions below with the types of microscopy Th

Match the descriptions below with the types of microscopy. There is one best match for each. _____ uses a light microscope with an optical component to take advantage of the different refractive indexes of light passing through different regions of the cell. phase contrast _____ employs a light microscope and requires that samples be fixed and stained in order to reveal cellular details. Transmission electron Co focal fluorescence _____ requires the use of two sets of filters. The first filter narrows the wavelength range that reaches the specimen and the second blocks out all wavelengths that pass back up to the eyepiece except for those emitted by the dye in the sample. scanning electron bright-field _____ scans the specimen with a focused laser beam to obtain a series of two-dimensional optical sections, which can be used to reconstruct an image of the specimen in three-dimensions. The laser excites a fluorescent dye molecule, and the emitted light from each illuminated point is captured through a pinhole and recorded by a detector. _____ has the ability to resolve cellular components a small as 2 nm. ______ requires coating the sample with a thin layer of a heavy metal to produce three-dimensional images of the surface of a sample

Solution

1)Phase contrast uses a light microscopewith an optical componant to take advantage of the different refractive indexes of light passes through differnt regions of cell.

2)Bright field employs a light microscope and requires that samples be fixed and stained in order to reveal cellular details.

3)Fluorescence requires the use of 2 sets of filters.

4) Confocal scans the specimen with a focused laser beam to obtain a series of 2D optical sections.

5)Transmission electron has the ability to resolve cellular componants a small as 2nm.

6)Scanning electron requires coating the samples with a thin layer of a heavy metal to produce 3D images of the surface of a sample.