Which of the following stabilizes the transition state oxyan

Which of the following stabilizes the transition state oxyanion that forms during chymotrypsin catalysis?

A:Hydrogen bonds from N-H groups

B:A covalent bond to histidine

C:Hydrophobic interactions in the hydrophobic pocket

-Reasoning behind your answer would be appreciated!

Solution

Correct answer is:A:Hydrogen bonds from N-H groups

Reason:Negative charge present on O atom in oxyanion is stabilized by interactions i.e by forming hydrogen bonds from NH groups of proteins.