Why might you wish to do a low stringency annealing temperat

Why might you wish to do a “low stringency” annealing temperature in a PCR reaction?

Solution

A \"low stringency\" condition permit hybridisation between nuclic acids where there are some mismatched bases.It is achieved by increasing NaCl concentration or decreasing annealing temperature below the T_m of the molecules or increasing formide concentration.
We might wish to do a “low stringency” annealing temperature in a PCR reaction when we are comparing DNA between different species or in experiments where a mismatch is expexted like searching for a single base mutation.