As bacteria cultures age and acquire cell wall damage how wi

As bacteria cultures age and acquire cell wall damage, how will the Gram-Staining of Gram-positive cells be affected?

The cells will have a stronger purple color

The cells will be more likely to lose the crystal violet stain during decolorization

The cells will be more likely to retain acid-fast stains

Safranin will no longer be able to bind to the bacteria

Solution

The cells will be more likely to loseb the crystal violet stain during decolorization, because with age and cell wall damage, techoic acid layer will be degrading, which retains the crystal violet stain and therefore if techoic acid layer will degrade, the gram positive bacteria willl start losing crystal violet.