If the agarose gel that you ran to see the bands was 08 and

If the agarose gel that you ran to see the bands was 0.8% and the DNA marker you used had markers from 1.0 kilobase pair to 24.0 kilobase pairs, would you be able to see all of the potential DNA fragments from the digestions? Why or why not?

What is competency for a cell? How is it achieved?

Solution

1.

Ideally for this range we should use 0.5 or 0.6 percent gel which is suitable for the range of 1000bp to 30000bp as they mentioned here the range of marker is 1-24 kb so by using 0.5 or 0.6 percent gel you can able to see all of the potential DNA fragments from the digestions. 0.8 is suitable for the range of 800 to 12000 bp.

2. Competency of a cell is a capacity of cell to take foreign DNA molecule. if the bacterial cell walls are altered so that DNA can pass through more easily. Such cells are said to be \"competent.\"Cells are made competent by a process that uses calcium chloride and heat shock.