Name at least 3 important factors to consider when developin

Name at least 3 important factors to consider when developing a lysis method using enzymes.

Solution

Lysis:Lysis means Losing..Breaking down of a membrane of a cell is called as lysis of cell membrane.

When developing a lysis method by using enzymes such method is called as enzymatic lysis.

Some factors influences the enzymatic lysis reactions such as Enzyme concentration,structure of the eczyme,enzyme specificity,temparature,PH,etc.

out of all these the following 3 factors are important factors when developing a lysis method using enzymes.

they are.. 1.Structure of a molecule.

2.Temparature.

3.PH

1. Structure of a molecule: a molecule structure influencing the lodging of a enzyme.The specific enzymes can bind at the binding site of a molecule.

2. Temparature:Enzyme adversly effected to the temparature.over a period of a time enzymes will be deactivated at even moderate temparature.

3. PH : Enzymes are effected by changes in the optimum PH.

extreamly high and low PH values generally result in complete loss of an activity of an enzyme.