5 Examine Figure 5 What conclusion can you draw from this gr

5. Examine Figure 5. What conclusion can you draw from this graph? Make sure to first look at the title and understand all abbreviations and your axes. Here are some definitions to help you out with Figure 5: A CFU or colony forming unit is a measurement of the number of culturable bacterial cells in a given sample. Those individual cells give rise to visible colonies when plated on an agar medium. These colonies are counted and incorporated into the following equation in order to estimate the CFUs (we will be using this equation later in the semester): of colonies x dilution fac CFU/g volume of culture plated or CFU/mL 6. Examine Figure 7. a. What kind of diagram is displayed in this figure? b. What are these types of diagrams used to show? c. What experimental methods in the lab were done to enable the used bioinformatics (ie. computer examination of DNA sequence analysis) to construct figure? (Hint: think about what you will be doing this semester for the River City Science project)

Solution

Conclusions for figure number 5.

The colony forming unit (CFU) did not show much difference between the sites and the values obtained are much lower as compared with the values obtained via FCM. The difference could be attributed to the fact that CFU could count only the viable cells while CFM could record the nonculturable bacteria as well.