Physical Biology of th Tf RNA polymerase s unit and B togeth

Physical Biology of th Tf RNA polymerase s unit and B together cons approximately 0 o o the total mass of protein in an E. coli cell, how many RNA polymerase molecules are there per cell assuming each B and B\'subunit within the cell is found in a (A) complete RNA polymerase molecule? The subunits have a mass of 150 kDa each. (5pt (b) Rifampin is an antibiotic 16S used to treat Mycobacterium 23S addition of rifampin infections such as tuberculosis It inhibits the initiation of (B) transcription, but not the elongation of RNA transcripts The time evolution ofan E. coli ribosomal RNA (rRNA) operon after addition of rifampin is shown in the figure (A)(c) An ron is a collection of genes (C) scribed as a single unit. U the figure to estimate the rate o transcript elongation. Use the beginning of the \"Christmas tree\" morphology on the left o Figure (A) as the starting po p) for transcription. (5 (e) Using the calculated 1 kb rate estimate t frequency of initiation of of the gst the most transcribed in E. coli. (5 p) genes are typical E coli cell has a division time of 3 uming there is no ribosome degradation, contains 20.0 thesizing rRNA at how many RNA polymerase molecules must be coli are involving in any instant? What percentage of the RNA polymerase molecules in E. transcribing rRNA genes? (5 pt) Smartphone Based colorimetry article.pdf A Pa

Solution

Figure B-C as mentioned in the legend shows the time course effect of rifampisin on the initiation of transcription. In Figure B , C as the effect of rifampisin is increased the transcription initiation by ribosomes is blocked. Hence less ribosamal subunits are present. When compared to C which was exposed to rifampisin for 70 sec, B has slightly higher number of ribosomes initaiting transcription as B is exposed for only 40 sec.