Why do you need to use a buffer while studying DNA by electr

Why do you need to use a buffer while studying DNA by electrophoresis

Solution

Answer:

Gel electrophoresis: It is an analytical technique used to separate the macromolecules such as the DNA, RNA and proteins. The separation of the molecules is based on the size. During the separation of molecules, small size molecules move faster than the large size molecules.

Agrose gel used for separation of the DNA and RNA

Polyacrylamide (PAGE) gels are used for separation of the Proteins.

Buffers are used to maintain the pH of the solution and prevents the dentauration of the DNA or it inhibits the DNAase activity. It is also required for conduction of the electric current by ions during the electrophoresis. Example: EDTA buffere and TAE buffer.