Consider the table and the figure then answer the following

Consider the table and the figure, then answer the following questions true or false High salt concentrations affect the accuracy of the Bradford assay Ideally, assays should be read between 5 and 20 minutes after preparation The Bradford assay is not suitable for protein solutions containing high detergent concentrations You make unknowns and standards, but read the unknowns an hour after you read the standards - as a result, you overestimate the unknown concentration.

Solution

a) High salt concentrations affect the accuracy of Bradford assay is false. This is because the protein samples usually contains salts, solvents, buffers, preservatives, and metal chelating agents. Using Bradford for detection of protein concentration is very advantageous against these molecules because they are compatible to each other and will not interfere.

b) Ideally assays should be read within 5 to 20 minutes after preparation is true. This is because Bradford assay is very rapid and the total time taken to set up and complete the assay is under 30 minutes.

c) The Bradford assay is not suitable for protein solutions containing high detergent concentrations is true. This is because the Bradford reagent is inhibited by the presence of detergents. But, this problem can be avoided by the addition of cyclodextrins to the assay mixture.

d) nknowns and standards are prepared but reading of unknown after one hour of reading standard may produce high unknown concentration is false. This is because the dye reagent is a stable ready to use product prepared in phosphoric acid. It can remain at room temperature for up to 2 weeks before its degradation. Therefore, delay in measuring of unknown concentration does affect the concentration.