PCR Requires DNA helicase to separate the template strands U

PCR:

Requires DNA helicase to separate the template strands.   

Utilizes either an upstream or downstream primer, but not both simultaneously.

Requires a large amount of template DNA to be successful.

Utilizes the DNA polymerase of a thermophilic bacterium.

Can only be used to amplify DNA if the sequence is precisely known.

Its not the first one.

Solution

PCR is an in vitro technique for rapidly synthesizing large quantities of a given DNA segment that involves separating the DNA into its two complementary strands, using DNA polymerase to synthesize two-stranded DNA from each single strand, and repeating the process used to amplify DNA if the sequence is precisely known.