Say you want to test the effect of fructose on the expressio

Say you want to test the effect of fructose on the expression of GFP in pGLO-transformed cells. You are given a 10 ml broth of LB with ampicillin, and a 10% fructose stock solution. How will you prepare the broth so that the final total volume remains 10 ml and the final concentration of fructose added is 0.3%?

I will remove...(answer with numbers/decimals only)

______ml of broth from the 10 ml LB/ampicillin tube, and replace it with

______ml of the 10% fructose stock.

Solution

you will remove 0.3ml ( 300 microlitres) from the 10mL LB/ampicillin tube and replace it with 0.3 mL of 10% fructose stock soln.