I was thinking 1020 what do you think they double every cycl

I was thinking 10^20 what do you think? they double every cycle.

PCR PCR is applied to a microtube solution that has about 10 copies of a target sequence. Assuming \"perfect\" efficiency, how many copies are present after cycles?

Solution

A PCR reaction is used to exponentially increase the number of target DNA copies using appropriate primer sequences. Every time a PCR reaction completes, the number of target DNA copies become double. So, if we start with a sample containing x number of DNA sequences, these will become x*x or x² after one cycle. These resultant sequences will be again amplified in next PCR cycle and will become x⁴ in number. Thus, after y number of cycles, the total number of DNA sequence copies will be x^y.

Thus, according to the question, a solution containing 10 DNA sequences, the number of target copies after 20 cycles will be 10²⁰.