Someone with a helicase enzyme defect needs therapy How woul

Someone with a helicase enzyme defect needs therapy. How would you design a therapy for the patient?

Part A: Specifically, what experiments would you use to study the helicase defect?

Part B: How would this mutation impact DNA replication in the patient?

Solution

Helicase enzyme plays a very important role in DNA replication. It unwinds the double stranded DNA into single stranded and allows the strands to be copied.DNA helicases involved in DNA repair and cancer. The therapies generally used for helicase enzyme defect is Recombinant DNA technology.

Part A: Gel electrophoresis and PAGE can used to study the helicase defect. DNA helicases forms a hexamer structure during DNA replication.Defect in the helicase enzyme disturb the structure.These experiments used to find the DNA lesions and find the hexamer formation.

Part B: The mutation in the helicase linked to cancer predisposition and premature aging disorders.Defect in DNA helicase that effect DNA replication add wrong neucleotides to the copy strand.And defect in RNA helicases linked to cancers, many infectious diseases and neuro-degenerative disorders.