A sample of an unknown peptide undergoes a single round of E

A sample of an unknown peptide undergoes a single round of Edman degredation, which liberates PTH-Gly. Another sample of the peptide is treated with trypsin, and a third sample is treated with chymotrypsin. The table below shows the fragments. Complete parts (a) and (b) below.

b) Write the sequence of the peptide.

Question 16 of 19 Tryptic fragments Chymotryptic fragments Lys-Asn-Tyr Phe-Ser Ala-Val-Thr-cys-Asp-Gln-Lys-Phe Gly-Pro-Phe-Lys Asn-Tyr-Ala-Val-Thr-Cys-Asp-Gln-Lys Ser Gly-Pro-Phe a) Line up the fragments below so that fragments with the same sequences overlap. Drag each fragment to the appropriate location. Ser Gly-Pro-Phe-Lys Phe-Ser Lys-Asn-Tyr Asn-Tyr-Ala-Val-Thr-Cys-Asp-Gln-Lys Gly-Pro-Phe Ala-Val-Thr-Cys-Asp-Gln-Lys-Phe Previous Give Up & View Solution Check Answer Next Exit

Solution

a)The overlap list of fragments is :

1)Ser

2)Phe-Ser

3)Gly-Pro-Phe-Lys

4)GLy-Pro-Phe

5)Lys-Asn-Tyr

6)Asn-Tyr-Ala-Val-Cys-Asp-Gin-Lys

7)Ala-Val-Thr-Cys-Asp-Gin-Lys-Phe

B)The sequence og the peptide is

H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr--Phe-Thr-Ser-Cys-OH