A Examine the Moon shaped enzyme on the left Decide with the

A. Examine the Moon shaped enzyme on the left. Decide with the two substrates A or B would be most likely to bind to the enzyme.

B. which of the substrates in the above question would have a lower Km.

I\'m sort of lost on how to do this part of the equation could I please get some help thank you

Solution

The moon shaped enzyme will bind to substrate B. This is because, the Lysine has a positive NH3+ group that binds to the negative charge on substrate B. The Glutamate has negative charge that binds to the + charge of substrate B. Trp and Gly will bind to CH3 by hydrogen binding. S

Substrate B will have low Km because it has high affinity. If the enzyme has high affinity towards a substrate then the km will be low