Describe an experiment to incorporate tyrosine instead of th

Describe an experiment to incorporate “tyrosine” instead of the normal stop codon “UAG” in E. coli.

Solution

Codon UAU and UAC codes for tyrosine.

Instead of stop codon (UAG), it should be either UAU or UAC.

tRNA^Tyr-TyrRS (tyrosyl-tRNA synthetase) pair from the archaeon Methanococcus jannaschii can be used to suppress the stop codon.