You started with a cell count of 65 x 105 cells ml in a tot

You started with a cell count of 6.5 x 10⁵ cells / ml in a total volume of 5ml. You need to seed a six-well plate with the following numbers. Calculate the volume of cell suspension you will need to transfer into 1 well. Each letter represents a different scenario.

a) 1.5 x 10⁴ cells/mL and up to 2 mL / well

b) 2.5 x 10⁵cells/well and up to 2 mL / well

c) 3 x 10⁵cells/well and up to 2 mL / well

Solution

Cell count of starting solution 6.5 x 10⁵

A) for 1.5 x 10⁴ /mL and 2 ml/well volume required = 0.52 mL

B) for 2.5 x 10⁵/well volume required = 0.385 mL

C) for 3 x 10⁵/well volume required = 0.461 mL