How would you identify isolate amplify and then clone the ge

How would you identify, isolate, amplify, and then clone the gene that encodes for a specific protein?

-Use knowledge of PCR, restriction enzymes, primers, vectors, RFLP, NCBI, genomes, and microarrays.

Solution

The basic purpose of gene isolation is to integrate it with the host cell to get the desired product usually desired protein. First of all we should know that gene isolation was made possible with the help of recombinant DNA technology. In the history first time gene was isolated from the bacteria with the help of bacteriophages. These phage can hybridize with the bacterial DNA. By isolating the phages we can isolate the gene. This is the fundamental principal of gene isolation. Bacteriophages infect the bacteria and phage DNA integrates with the bacterial DNA. This integrated phage DNA is known as prophage, and the bacterium is known as lysogen. Phage goes two forms of cycles, lysogenic and lytic cycle. Induction will bring about extraction of the prophage and its multiplication to make numerous progeny, i.e. it enters a lytic phase in which the bacteria get burst and destroyed. Extraction of prophage is not accurate, it always contain an additional gene or part of gene of bacteria DNA. When this phage attacks to the new host cell and it transfer the bacterial gene to the new bacteria. In this way a new gene or allele will incorporate to the host cell this process is known as transduction.