Describe in a detailed explanation for the following a Expla

Describe in a detailed explanation for the following:

a. Explain what is inferred by \"transformation of Arabidopsis by Agrobacterium\"

b. Explain the two different uses of the CaMV 35S promoter

Solution

\"Transformation of Arabidopsis by Agrobacterium\";

The Arabidopsis is highly susceptible to Agrobacterium-mediated transformation using flower dip and vacuum infiltratio protocols.Arabidopsis has also become a major system to investigate the mechanism of Agrobacterium mediated transformation of somatic tissue.

Assuming that a gene function is generally attributable to biochemical activity subcellular localization and interacting partners of its protein product,experimental assays are required to confirm every in silico prediction or to resolve ambiguous or uncertain predictions.

(b) Ans...Different uses of the CaMV 35S promoter;

Different uses of the CaMV 35S promoter are the promoter responsible for the transcription of the whole genome of a Cauliflower mosaic virus infecting turnips, The promoter was named CaMV 35S promoter because the coefficient of sedimentation of the viral transcript whose expression is naturally driven by this promoter is 35S.

The 35S promoter is a very strong constitutive promoter, causing high levels of gene expression in dicot plants,the 35S CaMV promoter is generally considered to be a strong constitutive promoter1 and it facilitates high level of RNA transcription in a wide variety of plants.