List some of the relative advantages and disadvantages of us

List some of the relative advantages and disadvantages of using X-ray crystallography and NMR spectroscopy to determine the structure of a protein.

Solution

Crystallography of proteins or biological molecules is a best technique, because obtaining well diffracting crystals is a difficult process.
The main differences between these two techniques are as follows..
NMR is limited to smaller proteins, while X-crystallography has no limit (difficulty increases with large complexes).
Protein dynamics can be studied using NMR, while X-ray crystallography gives static structural information.
Sample preparation, requirement are different.
No single technique is strong enough to address any biological problem.