Nutrient Broth Cultures Staphylococcus epidemidis Pseudomona

Nutrient Broth Cultures Staphylococcus epidemidis Pseudomonas aeruginosa Procedure: 1. Each team of four will culture all listed organisms. Assign each person a 2. different organism. organism for isolation on a plate of each of the six types of media. 3- assigned Label all plates well and place in the incubator for incubation at 37 C for 24-48 hours. esults: Indicate on the table below which organisms grew on which plates, the amount of growth occurring and other growth effects color, media changes, hemolysis, etc.) Escherchia TStaphylococcus Staphylococcus Pseudomona epidermidis Laeruginosa Nutrient agar PEA Blood agar ttt beta MacConkey pink colony in col. EMB M Black Mannitol Salt Verio medi Cetrimide PEA

Solution

7.a. No growth of staphylococcus aureus on macconkey agar indicates that it is a gram +ve organism

b.

Nutrient agar: creamcolor colonies

PEA: cream color colonies

Blood agar:  large, round, golden-yellow colonies with Beta hemolysis

Macconkey: no growth

EMB: Small, circular colonies

Mannitol salt agar: colonies are colour less and media changes from lite pink to yellow.

Cetrimide: no growth

8. a.No growth of staphylococcus epidermis on macconkey agar indicates that it is a gram +ve organism

b.

Nutrient agar: creamcolor colonies

PEA: cream color colonies

Blood agar: alpha  on blood agar

Macconkey: no growth hemolysis

EMB: Small, circular colonies

Mannitol salt agar: colonies are colour less and no change in colour of media.

Cetrimide: no growth

9.a. Growth of E.coli on emb and macconkey agar indicates that it is a gram -ve organism

b.

Nutrient agar: creamcolor colonies

PEA: growth is normal

Blood agar:  Gamma-hemolytic (nonhemolytic) colonies on blood agar

Macconkey: no growth

EMB: very dark color colonies

Mannitol salt agar: colonies are colour less and no change in colour of media.

Cetrimide: no growth

10.a. Growth on cetrimide agar indicates it is p.aeroginosa.

b.

Nutrient agar: Growth is normal

PEA: growth is normal

Blood agar:  Gamma-hemolytic (nonhemolytic) colonies on blood agar

Macconkey: colourless colonies

EMB: color less colonies

Mannitol salt agar: colonies are colour less and no change in colour of media.

Cetrimide: growth is observed